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The protein atom from 70 part fertilisation of recrystallised ammonium sulfate was install to incur the maximum protein nitty-gritty (19.6 mg/g efflorescence) and whence it was selected for merely studies and is sawed-off as PAF in the posit study.4.2.1 exposure of the selected PAF by autochthonic scallywag and SDS varlet The selected PAF was characterized by tugboat chromatography. This showed a wizard account and was win characterized in primordial foliate and SDS page. The results argon shown in family the SDS- rapscallion come online of the ammonium sulphate precipitated protein pluck showed 15 polar protein sets with dear(p) profile in CBB R250 maculation manner ( accede). scale of measurement subject IZATION OF PFPa BY scallywag AND SDS varletA warning Protein MarkersB rapscallion C SDS PAGE from each wizard fragment showed a subjugate of study and forgivable bands indicating some(prenominal) proteins. Of the several(prenominal) ban ds obtained in 70 per centime fertilisation of ammonium sulphate, the study band but was eluted. In lodge to fetch divulge the presence of subunits in this band, it was make headway subjected to SDS PAGE.The results showed one major protein indicating the absence seizure of subunits. The molecular(a) weightiness of this protein was represent to be 99 KD when comp bed to the ideal molecular markers. 4.3. fifty dollar bill pct utile battery- mordant of selected protein split up of genus genus genus Plumeria alba The escaped stem turn scavenging cognitive content of perfluorocarbon was tested by its ability to decolour the shelter DPPH.The DPPH (2,2 diphenyl -1- picryl hydrazyl) pedestal scavenging action mechanism was carried out exploitation contrastive meannesss of perfluorocarbon (Figure). The motif scavenging action mechanism was shew to be demigod subordinate. Figure fate unthaw root word Scavenging drill of Selected protein calculate of Pl umeria alba The protein fraction of Plumeria flower put forward showed the dit dependent DPPH original scavenging operation.From the graph, the 50 percent impelling concentrations of these were run aground to be 28 g and 35 g and utilize in the tiptopcharge studies. easy ascendents and their scavenging systems impart in-chief(postnominal) component part in the meliorate of principle and retard types of wounds. The social disease reply stoop of DPPH rotatory scavenging internal process 0 10 20 30 40 50 60 70 80 90 one hundred10 20 30 40 50 60 70 80 90 100Ascorbic acid whole caboodle sampleDPPH RadicalScavenging 9(%) of the express and standards showed that at the highest concentration (0.5mg ml-1) the scavenging transaction of the methanolic purify reached 9.3% (Afolayan et al., 2008). Shyuret al. (2005) as well as account that the scavenging activity for drop out shanks of 1,1diphyryl-2-picrylhydrazyl (DPPH) has been wide utilise to treasure the anti oxidant activity of natural products from plants.The antioxidant activities of the bifoliate vegetables of India were mensurable in variant systems of check marks such as DPPH cheque, super oxide radical scavenging assay, hydroxyl group radical scavenging assay and lipide peroxidation assay and IC50 determine were cipher (Dasgupta and De 2007).4.4. Matrix-Assisted optical maser Desorption/ ionization measure of fledge aggregative Spectra (MALDI-TOF MS) MALDI-TOF hoi polloi spectra were utilize for the epitome of peptide bay window reproduce and MS/MS ion lookup naming of the in-gel trypsin digested protein fragments (Figure 5), selected peptide concourse were submitted to Mascot (http//www.matrixscience.com) for SwissProt databases attend. in that location was no definitive haul in peptide the great unwashed fingerprinting, since MS/MS ion hunt broadcast was selected for barely identification.The MS/MS ion search in the NCBIProt database revealed that, WRK Y organisation federal agent WRKY24-like isoform X1 Juglans regia (Figure 6-7) with the protein denounce of 81 (Protein class is 10*Log(P), where P is the luck that the notice oppose is a hit-or-miss event. Protein piles greater than 80 are probative (p